Seymour is a town located in the Southern end of the Goulburn Valley in the Shire of Mitchell, Victoria, Australia and is located 104 kilometres (65 mi) north of Melbourne. At the 2021 census, Seymour had a population of 6,569. The township services the surrounding agricultural industries (primarily equine, cattle, sheep and wine) as well as the nearby military base of Puckapunyal (population 1,176), which is an important training centre for the Australian Army. Other important sectors of employment in Seymour include retail, light engineering, agricultural services support, medical services, and education.

Seymour is located adjacent to the junction of the Hume and Goulburn Valley Highway. The Hume Highway crossed the Goulburn River and diverted all Melbourne–Sydney bound traffic though the township for over 100 years until a freeway bypass was opened routing all non-local and interstate traffic around the township in 1982.